Production of tpa using eukaryotic n prokaryotic cells Essay

Production of tpa using eukaryotic n prokaryotic cells - Essay Example coli (prokaryotic). This is because of the high cost of the purification scheme required on the tPA produced from E. coli. That from the mammalian cells does not require lots of purifications because the cell environment and content is similar to that of the body cells (in vitro). tPA produced in E. coli required more purification to acquire the required state because it is composed of many components that are naturally not found with tPA in vitro conditions. The contents produced together with tPA in mammalian cells are similar to those in vitro hence are not foreign materials resulting to a less purification process. The E. coli based production process utilizes approximately sixteen purification steps (downstream) as compared to the five purification steps in CHO. Since the purification process in mammalian cells is not that complex less operating labour and supervision is required compared to the E. coli based. The CHO process utilizes nine percent of its total cost on operating labour while E. coli based utilizes twenty two percent. Also, the cost of acquiring the different purification units required for the many purification stages is higher in the E. coli based. Mammalian based process utilizes only twenty five percent of the total production cost on recovery (purification) compare to eighty eight percent on E. coli based. For the solubilization and renaturation step in the E. coli based production, approximately five tonnes of urea and twenty-six tonnes of guanidine are required. The bacteria lack efficient secretion mechanisms for tPA, therefore, the proteins are deposited as inclusion bodies. For the recovery of the proteins, a renaturation procedure is required. Since the proteins in the bacteria are produced in low concentrations (about 2.4 mg per litre) the practicability of a renaturation based production process is limited. The E. coli based production does yield to larger quantities of products that

Project environment Essay Example | Topics and Well Written Essays - 3500 words

Project environment - Essay Example In this scenario, the accomplishment of novel technology based infrastructure is intended to improve the general company as well as its operations management processes. I will present a complete outline of key tasks and areas those are considered to be problematic from the diagnosis point of view. Moreover, I will also propose the possible changes and improvements that we are able to carry out throughout new technology infrastructure. At the current time the businesses are facing a lot of problems regarding effective business projects management and reduced cycle time. In this regard, they are facing considerable delays between the project plan and actual implementation. Therefore, businesses are requiring to frequently adjusting company operations to survive in the everlasting competitive market situations. Additionally, to productively handle and manage new information and maintain it all through the corporation is critical to deal with these challenges. Thus, steady, business-wide knowledge sharing has, as a result, turned out to be essential. In this scenario, businesses apply solutions to collect, manage, and share knowledge. The most common opening application has been in the field of learning and training. Unluckily, usual classroom training plans are very time-consuming and costly to help businesses to tackle out mission-critical information quickly and competently (OneTouch, 2009). According to Efstathiades, Tassou, Antoniou, & Oxinos (1998) companies whose continued existence depends on upholding new, up to date, and well-informed business information need a flexible information sharing platform. Therefore, the business-wide knowledge sharing is one of most important factors. In scenario of latest technology implementation at a company there is need to change or update a lot of factors and business elements. In addition, there are a lot of issues involved in company management and